Liquid Chromatography (Virginia Tech)

• INTRODUCTION
  • Brief description of HPLC history, principles and instrumentation.
• GENERAL THEORY
  • Comprehensive presentation of basic HPLC theory, kinetics (band broadening), and thermodynamics of adsorption from solutions.
• ADSORBENTS
  • Basic description of the HPLC adsorbents structure, surface chemistry, bonded phases, and major parameters of packing materials.
• REVERSED-PHASE HPLC
  • Comprehensive description of the most popular HPLC mode. Retention mechanism and influence of the bonded phase type and density, mobile phase composition and other parameters are described.
• INSTRUMENTATION
  • Full description of the major components of HPLC system. All ypes of pumps, injectors, connectors, etc., including schematics and explanation of basic principles and maintenance are presented.
• DETECTORS
  • RI, UV, Diode Array, Fluorescence, LC/MS, ELS and other HPLC detectors are comprehensively described. Selected applications explaining benefits of each detector are shown.
• HPLC GLOSSARY
  • More than hundred basic HPLC terms with brief explanation are shown. Highly interlinked with the main text.